BSIS012078:2006 ISO INTERNATIONAL STANDARD 12078 IDF 159 First edition 2006-02-01 Anhydrous milk fat Determination of sterol composition by gas liquid chromatography (Reference method) Matieres grasses laitieres anhydres-Determination de la composition steroliqueparchromatographie enphase gazeuse (Methodede reférence) Reference numbers ISO 12078:2006(E) IDF 159:2006(E) = BS ISO 12078:2006 , Uncontrolled Copy, (c) BSI Contents Page Foreword.. Foreword 1 Scope 2 Normative references 3 Terms and definitions 4 Principle. GMT+00:00 2006, 5 Reagents. 6 Apparatus. 7 Sampling. 8 Preparation of test sample.... .6 9 Procedure .... 9.1 Sterolstandard solutions 27 03:46:26 9.1.1 Calibrating solution for the determination of cholesterol response factor... 9.1.2 Qualitative solution for the determination of sterol retention time.. .6 9.2 Test portion... 9.3 Saponification.. 9.4 Extractionoftheunsaponifiablematter. 9.5 Thin-layer chromatography (TLC)...... Licensed Copy: sheffieldun sheffieldun, na, Mon Nov 2 9.5.1 Preparationof TLCplates.. 9.5.2 Preparation of glass plate-developing tank... 9.5.3 TLC of unsaponifiable matter.... 9.6 Preparation of silyl derivatives... 9.7 Qualitative analysis 9.8 Quantitative analysis.. 9.8.1 Calculation of response factor.... .8 9.8.2 Determinationofthetestportion. 9.8.3 Calculationandexpressionofresults. 10 Precision... 10 10.1 Interlaboratorytest .10 10.2 Repeatability.... 10.3 Reproducibility... ...10 11 Test report. .11 Annex A(informative)Examples of thegas-liquid chromatographic analysis.. ...12 Annex B (informative)Results of an interlaboratorytest... ..14 Bibliography .. ili BSIS012078:2006 , (c) BSI Foreword Copy, Iso (the International Organization for Standardization) is a worldwide federation of national standards bodies (Iso member bodies). The work of preparing International Standards is normally carried out through Uncontrolled and non-go overnmental, in liaison with IsO, also take part in the work. IsO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/lEC Directives, Part 2 GMT+00:00 2006, The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent ISO12078|iDF159waspreparedbyTechnicalCommitteeISO/TC34,Foodproducts,SubcommitteeSC5, Licensed Copy: sheffieldun sheffieldun, na, Mon Nov 27 03:46:26 IDF. iv BSIS012078:2006 Foreword IDF (the International Dairy Federation) is a worldwide federation of the dairy sector with a National Standing Committees carrying out the technical work. IDF collaborates with IsO in the development of standard methods of analysis and sampling for milk and milk products. Draft International Standards adopted by the Action Teams and Standing Committees are circulated to the National Committees for voting.Publicationas an International Standard requires approval byat least5o % of theIDFNational Committees casting avote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. IDF shall not be held responsible for identifying any or all such patent rights ISO 12078liDF159 was prepared by the International Dairy Federation (IDF)and Technical Committee ISO/TC 34, Food products, Subcommittee SC 5, Milk and milk products. It is being published jointly by IDF andiso. All work was carried out by the Joint ISO-IDF Action Team on Fat, of the Standing Committee on Main blank BSISO12078:2006 Anhydrous milk fat Determination of sterol composition by gas liquid chromatography (Reference method) WARNING-The use o